Jobs at Aera Therapeutics

Reporting to the Director of Molecular Biology and Automation, you will design, build, and test mRNA to support mRNA platform advancement and discovery programs. You will collaborate with Therapeutics Discovery and Analytical Sciences teams to support advancement of therapeutic mRNAs. You will require expertise at developing in vitro assays aimed at understanding fundamental concepts of mRNA biology including stability and maximizing protein expression. You will be working at our Cambridge office five days per day. 

• Design and clone mRNA expression constructs, including template assembly and sequence verification, in vitro transcription, and mRNA purification
• Transfect mammalian cell lines and primary cells and quantify protein expression by western blot, ELISA, or flow cytometry
• Develop and automate in vitro assays on liquid-handling platforms to increase assay throughput, consistency, and the pace of mRNA design iteration
• Integrate data across molecular, cellular, and in vivo assays to build a coherent view of what sequence features are driving performance
• Serve as the mRNA design representative on cross-functional program teams, driving alignment with delivery, analytical development and CMC
• Maintain records of all relevant data in the electronic laboratory notebook system and present your own data clearly at team meeting

• BS (8+ years experience), MS (6+ years experience), or PhD (2+ years experience) in an engineering or life sciences discipline
• Demonstrated hands-on molecular biology skills: PCR, molecular cloning (restriction and Gibson/HiFi assembly), plasmid prep, gel and capillary electrophoresis, Sanger sequencing analysis
• Experience with RNA handling techniques and an understanding of the practical requirements of an RNase-free workflow
• Experience adapting or developing assays on automated liquid-handling platforms (e.g., Hamilton, Tecan, Opentrons) to enable higher-throughput screening 
• Mammalian cell culture and transfection experience
• Working knowledge of the relationship between mRNA sequence features and protein expression outcomes
• Commitment to our Core Values: Respect, Integrity, Excellence, Impact